Internet
December 25, 2004
Oregon State University
biology.science.oregonstate.edu
Biology Program Information
West Nile Virus in the United States
– Yellow Fever. Gottfried Helnwein. Leid macht stark 1974 ... Gottfried Helnwein. Der höhnische Arzt (The Mocking Physician)1973 ... Family: Flaviviridae - Pestiviruses – Bovine viral diarrhea viruses 1 & 2 (BVDV- 1, BVDV-2) – Hog Cholera/classical swine fever (CSFV) • Hapaciviruses – Hepatitis C virus (HCV) • Flaviviruses – Rio Bravo – TBEV (tick-borne encephalitis) – JE (Japanese Encephalitis) – Dengue (types 1-4) – Yellow Fever
Leid macht stark
watercolor on cardboard, 1974, 73 x 48 cm / 28 x 18''
Family: Flaviviridae - Pestiviruses

– Bovine viral diarrhea viruses 1 & 2 (BVDV-
1, BVDV-2)
– Hog Cholera/classical swine fever (CSFV)
• Hapaciviruses
– Hepatitis C virus (HCV)
• Flaviviruses
– Rio Bravo
– TBEV (tick-borne encephalitis)
– JE (Japanese Encephalitis)
– Dengue (types 1-4)
– Yellow Fever
Gottfried Helnwein
Leid macht stark 1974
Flaviviridae Family Phylogeny
Flavivirus genus (visible version)
Primary flavivirus transmission
vector is the Culex mosquito
General life cycle of genus Flavivirus viruses
Flaviviruses are (+) sense RNA viruses with
no nucleoinvasive replication processes.
Flavivirus Genome and Translated
Protein Coding Regions
Conserved RNA Structures in general Flavivirus genome
*from Annu. Rev. Microbiol. 2002 56:371-402
Flavivirus Nucleocapsid
• Map indicates states reporting laboratory-positive
West Nile virus infection in birds, mosquitoes,
animals, or humans during 2002.
West Nile Virus in the United States as of February 25, 2004*

Map indicates human cases (in red) during 2003 until Feb. 25, 2004.

Colorado highest w/ 2477 cases, Nebraska next w/ 1867 cases.
WNV phylogenetic tree based on E-glycoprotein
nucleic acid sequence data (255 base pairs).
Gottfried Helnwein
Der höhnische Arzt (The Mocking Physician)1973
Gottfried Helnwein
Guten Morgen, Du Sau! (Good Morning, You Bastard!) 1972
Gottfried Helnwein, Earthquake, 1977
WNV Genome Structure

The genome is not segmented and consists of a single molecule of linear positive-
sense single-stranded RNA. The complete genome is 10488–10976 nucleotides long.
The 5'–end of the genome has a cap methylated nucleotide cap. The 3'–terminus has
no poly (A) tract, or a poly (A) tract (in case of some strains of tick-borne encephalitis
complex of flaviviruses).
*
Büchen-Osmond, C. (Ed), (2003). 00.026.0.01. Flavivirus. In: ICTVdB - The Universal Virus Database, version 3.
ICTVdB Management, The Earth Institute, Biosphere 2 Center, Columbia University, Oracle, AZ, USA
Replication of WNV in Host Cell
*from Annu. Rev. Microbiol. 2002 56:371-402
Electron micrographs of West Nile virus isolated from brain tissue
from a crow found in New York
*from CDC Website
Common Symptoms of WNV
Beautiful Victim I
watercolor on cardboard, 1974, 53 x 73 cm / 20 x 28''




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