Family: Flaviviridae - Pestiviruses
– Bovine viral diarrhea viruses 1 & 2 (BVDV-1, BVDV-2)– Hog Cholera/classical swine fever (CSFV)• Hapaciviruses– Hepatitis C virus (HCV)• Flaviviruses– Rio Bravo– TBEV (tick-borne encephalitis)– JE (Japanese Encephalitis)– Dengue (types 1-4)– Yellow Fever
Gottfried HelnweinLeid macht stark 1974
Flaviviridae Family PhylogenyFlavivirus genus (visible version)Primary flavivirus transmissionvector is the Culex mosquitoGeneral life cycle of genus Flavivirus virusesFlaviviruses are (+) sense RNA viruses withno nucleoinvasive replication processes.Flavivirus Genome and TranslatedProtein Coding RegionsConserved RNA Structures in general Flavivirus genome*from Annu. Rev. Microbiol. 2002 56:371-402Flavivirus Nucleocapsid• Map indicates states reporting laboratory-positiveWest Nile virus infection in birds, mosquitoes,animals, or humans during 2002.West Nile Virus in the United States as of February 25, 2004*•Map indicates human cases (in red) during 2003 until Feb. 25, 2004.•Colorado highest w/ 2477 cases, Nebraska next w/ 1867 cases.WNV phylogenetic tree based on E-glycoproteinnucleic acid sequence data (255 base pairs).
Gottfried HelnweinDer höhnische Arzt (The Mocking Physician)1973
Gottfried HelnweinGuten Morgen, Du Sau! (Good Morning, You Bastard!) 1972Gottfried Helnwein, Earthquake, 1977
WNV Genome Structure•The genome is not segmented and consists of a single molecule of linear positive-sense single-stranded RNA. The complete genome is 10488–10976 nucleotides long.The 5'–end of the genome has a cap methylated nucleotide cap. The 3'–terminus hasno poly (A) tract, or a poly (A) tract (in case of some strains of tick-borne encephalitiscomplex of flaviviruses).*Büchen-Osmond, C. (Ed), (2003). 00.026.0.01. Flavivirus. In: ICTVdB - The Universal Virus Database, version 3.ICTVdB Management, The Earth Institute, Biosphere 2 Center, Columbia University, Oracle, AZ, USAReplication of WNV in Host Cell*from Annu. Rev. Microbiol. 2002 56:371-402Electron micrographs of West Nile virus isolated from brain tissuefrom a crow found in New York*from CDC WebsiteCommon Symptoms of WNV
